By Springer-Verlag, Jorg Hoheisel
ISBN-10: 3540432159
ISBN-13: 9783540432159
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Because SNPs are the most common source of variation between individuals, they serve not only as landmarks to create dense genome maps, but also as markers for linkage and loss of heterozygosity studies. Large numbers of publicly available SNPs – over one million to date – have been found using dideoxy sequencing as well as mutation detection arrays [41–43]. In addition to mutation detection arrays, at least two other types of oligonucleotide arrays can be used for SNP analysis. The “HuSNP” assay allows nearly 1500 SNP-containing regions of the human genome to be amplified in just 24 multiplex PCRs and then hybridized to a single HuSNP array.
54 Heteroduplex Formation . . . . . . . . . . . . 55 A Study of the Secondary Structure of Nucleic Acids with Modified Bases . . . . . . . . . . . . . . 55 5 Concluding Remarks . . . . . . . . . . . . . 56 6 References . . . . . . . . . . . . . . . . 56 1 Introduction Scanning arrays are made by combinatorial in situ synthesis of a large number of oligonucleotides on a solid support in a spatially addressable fashion. They comprise sets of oligonucleotides of various lengths in which a series of oligonucleotides, complementary to the target RNA or DNA, is made by sequential coupling of nucleotides to a solid substrate (such as glass or polypropylene) that has been modified to allow oligonucleotide synthesis.
54 Heteroduplex Formation . . . . . . . . . . . . 55 A Study of the Secondary Structure of Nucleic Acids with Modified Bases . . . . . . . . . . . . . . 55 5 Concluding Remarks . . . . . . . . . . . . . 56 6 References . . . . . . . . . . . . . . . . 56 1 Introduction Scanning arrays are made by combinatorial in situ synthesis of a large number of oligonucleotides on a solid support in a spatially addressable fashion. They comprise sets of oligonucleotides of various lengths in which a series of oligonucleotides, complementary to the target RNA or DNA, is made by sequential coupling of nucleotides to a solid substrate (such as glass or polypropylene) that has been modified to allow oligonucleotide synthesis.