By Bogdanske, John J.; Hubbard-Van Stelle, Scott; Rankin Riley, Margaret; Schiffman, Beth M
ISBN-10: 1482282194
ISBN-13: 9781482282191
The texts, Laboratory Rat and Laboratory Mouse Procedural ideas guide and DVD are brief, simply read/watched manuals that assessment the very easy dealing with for the animal care technician throughout the transparent use of debate and accompanying photos, more advantageous with a transparent, comprehensible DVD demonstrating each one technique mentioned. not just is the protection of the laboratory specimen of shock, yet even more Read more...
summary: The texts, Laboratory Rat and Laboratory Mouse Procedural options handbook and DVD are brief, simply read/watched manuals that evaluation the very easy dealing with for the animal care technician throughout the transparent use of dialogue and accompanying photos, improved with a transparent, comprehensible DVD demonstrating each one approach mentioned. not just is the security of the laboratory specimen of shock, yet much more importantly, the protection of the human laboratory technicians and researchers. The reader is believed to have basic laboratory rodent education in position. this isn't intended as a main
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Additional resources for Laboratory Rat Procedural Techniques : Manual and DVD
Example text
To allow for an adequate recovery period, we further recommend that the maximum amount be withdrawn only once every 2 weeks (based on 10%). Daily samples can be collected as long as the cumulative blood volume does not exceed the calculated maximum over a 7-day period. Recommended skills: Basic handling and restraint of a rat. Know how to properly use an anesthesia machine if rats will be anesthetized. 2 × 75 mm L), blood collection tube, exam gloves, 2 × 2 gauze, petroleum jelly or artificial tears, anesthesia machine, and a tourniquet.
To allow for an adequate recovery period, we further recommend that the maximum amount be withdrawn only once every 2 weeks (based on 10%). Daily samples can be collected as long as the cumulative blood volume does not exceed the calculated maximum over a 7-day period. Recommended skills: You must know and understand the proper method to restrain a rat for this technique. The ability to operate a syringe and needle with one hand is necessary. Knowledge and experience of using an anesthesia machine are also helpful.
The ointment will allow the blood to bead up on the skin. Using a 27-gauge needle, gently poke into the vein, taking care not to pierce completely through it because this will result in blood accumulating under the skin. As the blood beads up, use a heparinized capillary tube to collect it. When finished, release the tourniquet and apply pressure with gauze to stop the bleeding. 19 20 Laboratory Rat Procedural Techniques: Manual and DVD recommended supplies • Heparinized capillary tube • Clippers • Artificial tears or petroleum jelly • Tourniquet • 27-gauge needle • Gauze Voice-Over 1: Clip the lateral surface of the rat’s hind leg to expose the saphenous vein.