By Sue Ann Wercinski
ISBN-10: 0824770587
ISBN-13: 9780824770587
An evidence of confirmed tools of chemical research, concentrating on the myriad purposes of stable part microextraction (SPME) to laboratories appearing high-sample throughput, quickly pattern turnaround time, low detection degrees, and soiled pattern matrices. It provides statement on advancements in SPME expertise from its inventor, Janusz Pawliszyn.
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Additional info for Solid phase microextraction: a practical guide
Example text
Requires no bench space. FeaturePurge and TrapSPMESample matricesAqueous liquids and solidsAqueous liquids, solids, gas (rarely)Sample pretreatmentNone for many samples. Matrix modifiers sometimes used such as salting out or adjusting pH. None for many samples. Matrix modifiers sometimes used such as salting out or adjusting pH. Lower capital cost for dual function hardware (liquid injection and SPME) and lower costs for maintaining and trouble-shooting hardware. Requires no bench space. No problems with foaming samples.
For large sample volumes (>5 mL), the amount of analyte absorbed by the fiber coating at equilibrium is directly proportional to the initial aqueous concentration, Co. The following equation is used when the volume of the aqueous sample, Vs, is much larger that the stationary phase volume; that is, its volume is relatively infinite to the fiber volume (Vs>KsV). 4) Where: n is the amount extracted by the fiber coating. Here, the sample volume does not need to be known (it is relatively infinite), which is ideal for field sampling and simplifies laboratory operations.
14, except for distribution constants. The curves represent: (A) p-xlyene, Ks = 831; (B) toluene, Ks = 294; and (C) benzene, Ks = 125. (From Ref. 5) Extraction Speed For Direct And Headspace Sampling. Speed of Extraction for Direct Sampling The speed of extraction is determined by how long it takes the analytes to move from the sample matrix to the fiber. This process involves: (1) the rate the analytes desorb from a solid surface if particulate matter is present, (2) the analytes migrating through the air or liquid sample, and (3) the analytes diffusing into the fiber coating [1].